Differentiation between commercial wine samples using fluorescence spectroscopy and multivariate analysis

Abstract

Steady state fluorescence spectroscopy in combination with Multivariate Curve Resolution – Alternating Least Squares (MCR-ALS) for spectral analysis was used to differentiate between commercial samples of wines available on the market. Wine trade marks from two Serbian producers that contain only one wine type were chosen, since such an approach is a starting step for further analyses of more complex samples containing different wine types. We also studied changes in the emission spectra of these samples over a period of seven days after opening the wine bottle. The emission spectra were recorded in the wavelength range 275 - 500 nm, after excitation in the 255 - 300 nm range. The spectra of the wine samples obtained from the same producer are very similar, i.e. they contain the same components at similar ratios. The changes in spectral components at 315 nm and 430 nm were the basis for differentiation between the wine samples obtained from the two producers, as well as for estimation of wine stability over time after the bottle opening. The results indicate that this may be a useful approach to fingerprinting wines from various producers, as well as to screening the stability of wine.