Investigation of the relationship between physiological traits and protein markers in soybean cultivars (Glysin max L.)

Abstract

This study was conducted to evaluate salinity tolerance in the seedling stage of soybean (Glysin max L.). A factorial experiment was performed in a randomized complete block design with three replications. Seventeen soybean genotypes were used at 3 salinity stress levels (consisting of control, 75 mM and 150 mM NaCl stresses). The experiment was carried out in greenhouse conditions and proline, sodium, potassium, and chlorophyll a, chlorophyll b, chlorophyll a/b and total chlorophyll content were examined. To create salinity stress, NaCI was used in the experiment. The results revealed that different salinity stresses had significant effects on all traits except for chlorophyll b and chlorophyll a/b. The cluster analysis in the control and at 75 and 150 mM salinity levels classified genotypes into two, two and three groups respectively. In each condition, the dpx and clean genotypes were placed in a group whose average traits were higher than those of the other genotypes. This can be generalized to the conditions of the control as well as of 75 and 150 mM salinity stress. Regression analysis showed possible informative loci encoding protein markers as a probable potential for selection strategies for salt conditions, as proved by complementary tests.