The identification of D-genome in some spring triticale lines

Abstract

Main problems limiting spring triticale cultivation are poor grain quality, plant height, low plant productivity and preharvest sprouting. One of the suitable way to improve this situation is application of wheat D-genome into triticale. During the secondary spring hexaploid triticale development it is available to observe the genomic reorganization and elimination of D-genome material. In order to resolve the problem regarding the identification of breeding samples bearing D-genome we carried out PCR analysis with chromosome specific SSR-markers and specific STS-marker of locus Sec2 (2RS). Further for precise identification of triticale lines 2R/2D-substituted we applied genomic in situ hybridization (GISH). So it has been shown that 7 lines of spring triticale (of 15 ones) bear 2R/2D substitution, results derived from SSR-PCR and STS-PCR analysis being approved by GISH. It means that PCR-analysis alone is enough to select D-genome bearing plants. Spring triticale forms L 8-4 and L 8-6 are segregating in rye chromosome number, among them there being 2R/2D-substituted, non-substituted plants and hybrids between them. In this respect, it is necessary to make individual selection based on the molecular marker application. Such amazing large ratio of 2R/2Dsubstituted forms in the collection investigated may be explained by the preliminary selection under field conditions according to low plant height and short growing period. Spring triticale forms appeared to be 2R/2D substituted are involved into breeding process.