Fluorometric determination of hesperidin in orange juices available on Serbian market

Authors

  • Leposava Pavun Faculty of Pharmacy, University of Belgrade, Serbia
  • Milena Jelikić Stankov Faculty of Pharmacy, University of Belgrade, Serbia
  • Dušan Malešev Faculty of Pharmacy, University of Belgrade, Serbia
  • Snežana Uskoković-Marković Faculty of Pharmacy, University of Belgrade, Serbia
  • Jasmina Dimitrić Marković Faculty of Physical Chemistry, University of Belgrade, Serbia
  • Predrag Đurđević Faculty of Sciences, University of Kragujevac, Serbia
  • Daniela Đikanović Centre for Multidisciplinary Studies, University of Belgrade, Serbia

Keywords:

hesperidin, orange juice, spectrofluorometry

Abstract

The spectrofluorometric method based on fluorescence ability of aluminium – hesperidin complex for the determination of hesperidin in orange juice is proposed. The linearity range of hesperidin in methanolic-aqueous solution was 0.08 – 18.0 μg/mL with LOD and LOQ values as 0.023 μg/mL and 0.070 μg/mL, respectively, and recovery values in the range 97.8 – 99.7%. Method was simplified by omitting any of surphactant agents usually applied in similar procedures, and successfully applied for the determination of hesperidin in orange juices commercially available on the Serbian market. 

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Published

06.02.2026

Issue

Vol. 17 No. 34 (2012)

Section

Articles