Cloning and characterization of Nbs-Lrr class resistance-gene analogs sequences in sunflower

Authors

  • V.S. Shevelukha Moscow Timiryazev Agricultural Academy, Department of Biotechnology, Russia
  • M.Y. Kuklev Moscow Timiryazev Agricultural Academy, Department of Biotechnology, Russia
  • Gennady I. Karlov Moscow Timiryazev Agricultural Academy, Department of Biotechnology, Russia

Keywords:

disease resistance genes, sunflower, Helianthus argofillus, resistancegene analogs, molecular marker

Abstract

A number of disease resistance gene-like DNA sequences were cloned from wild species of sunflower (Helianthus argofillus), using a PCR approach with degenerate primers designed from conserved NBS (nucleotide-binding site) motifs found in a number of plant resistance genes. After sequencing of cloned fragment, the similarity to the known resistance genes was found (NBS2, NBS5 – NBS-LRR class resistance-gene analogs in sunflower (Helianthus annuus), RLG5, c27 – resistance-gene homologues in soybean (Glycine max), FRGA-A5 – NBS-LRR resistance-gene analog in apple-tree (Malus domestica). These results indicate that resistance-gene analogs sequences amplified with the NBS-derived degenerate primers are valuable sources for developing markers in disease resistance-gene tagging, mapping and cloning

References

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Published

06.02.2026

Issue

Vol. 8 No. 15 (2003)

Section

Articles