Determination of genetic diversity among different tomato varieties using SSR markers

Authors

  • Elizabeta Miskoska - Milevska Faculty of Agricultural Sciences and Food, Food Institute, Department of Food Quality and Safety, University "Ss Cyril and Methodius", Skopje, Macedonia
  • Zoran T. Popovski Faculty of Agricultural Sciences and Food, Institute of Animal Biotechnology, Department of Biochemistry and Genetic Engineering, University "Ss Cyril and Methodius", Skopje, Macedonia
  • Blagica R. Dimitrievska Faculty of Agricultural Sciences and Food, Institute of Animal Biotechnology, Department of Biochemistry and Genetic Engineering, University "Ss Cyril and Methodius", Skopje, Macedonia
  • Katerina Bandzo Faculty of Agricultural Sciences and Food, Institute of Animal Biotechnology, Department of Biochemistry and Genetic Engineering, University "Ss Cyril and Methodius", Skopje, Macedonia
  • Koco D. Porcu Faculty of Agricultural Sciences and Food, Institute of Animal Biotechnology, Livestock Department, University "Ss Cyril and Methodius", Skopje, Macedonia

Keywords:

Lycopersicon esculentum Mill., varieties, SSR, genetic diversity

Abstract

The simple sequence repeats (SSR) have become the preferred molecular markers for variety identification, genetic mapping and phylogenic analysis. The aim of this study was to evaluate genetic relationships among six morphologically different tomato varieties (var. grandifolium, var. cerasiforme (red), var. cerasiforme (yellow), var. pruniforme, var. pyriforme and var. racemigerum) using nine SSR markers (LECH13, LE21085, LEMDDNa, LEEF1Aa, LELEUZIP, LE20592, TMS9, LE2A11 and LECHSOD). Fragment analysis was conducted using an Applied Biosystems DNA analyzer (ABI 3130) and GeneMapper® Software program. The data were analyzed using Power Marker Software and MEGA3 programs. The results showed that the lowest genetic distance (16.7415) was identified between var. cerasiforme (yellow) and var. cerasiforme (red), and the largest (34.9859) between var. pyriforme and var. grandifolium. On the other hand, the lowest genetic distance (22.1446) was observed between subsp. subspontaneum and subsp. spontaneum, and the largest (29.7147) between subsp. subspontaneum and subsp. cultum. Precise dendrograms were created based on the statistical data.  

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Published

06.02.2026

Issue

Vol. 16 No. 31 (2011)

Section

Articles